RESUMO: Tomando a província de Henan como exemplo, o sistema de índices de evasão rural foi construído a partir de três aspectos da economia, população e terra. Utilizando o método de peso de entropia, método de índice de vazamento, análise de autocorrelação espacial e Geo detector para analisar características de evolução espaço-temporal e fatores de influência do esvaziamento rural do município de 2000 a 2013 na província de Henan. Os resultados mostraram que a lacuna de esvaziamento rural entre condados na província de Henan foi expandida inicialmente e em seguida diminuiu, e toda a província estava no nível médio de evasão rural, e, além disso, o desenvolvimento da evasão rural na província de Henan era estável. As características de variação espacial de diferentes graus de evasão rural durante 2000-2013 eram óbvias, e o padrão espacial geral era mais alto na direção noroeste-sudeste e mais baixo na direção nordeste-sudoeste. Houve forte correlação positiva espacial no êxodo rural, e a aglomeração espacial foi levemente enfraquecida; o número de municípios nas áreas de aglomeração H-H e L-L era grande e as características de aglomeração eram óbvias; as áreas de aglomeração de H-L eram do zero com um pequeno número e distribuição dispersa. A influência de diferentes fatores no esvaziamento rural foi diferente, e a área de propriedade per capita, a proporção de indústrias secundárias e terciárias, o investimento em ativos fixos e a renda líquida per capita dos agricultores foram os fatores dominantes.

ABSTRACT: Taking Henan province as an example, the index system of rural hollowing was constructed from three aspects of economy, population and land. Using the entropy weight method, hollowing index method, spatial autocorrelation analysis and Geo detector, spatial-temporal evolution characteristics and influencing factors of county rural hollowing from 2000 to 2013 in Henan province were analyzed. Results showed that the gap of rural hollowing between counties in Henan province expanded first and then decrease, and the whole province was at the middle level of rural hollowing, so the development of rural hollowing in Henan province was stable and positive. Spatial variation characteristics of different degrees rural hollowing during 2000-2013 were obvious, and the overall spatial pattern was higher in the northwest-southeast direction and lower in the northeast-southwest direction. There was a strong spatial positive correlation in rural hollowing, and spatial agglomeration was slightly weakened; the number of counties in H-H and L-L agglomeration areas was large and its agglomeration characteristics were obvious; the H-L agglomeration areas started from zero with a small number and scattered distribution. Influence of different factors on rural hollowing was quite different, and the per capita homestead area, the proportion of secondary and tertiary industries, the investment in fixed assets and the per capita net income of farmers were the dominant factors.

Mucopolysaccharidosis type I (MPS I) arises from a deficiency in the α-L-iduronidase (IDUA) enzyme. Although the clinical spectrum in MPS I patients is continuous, it was possible to recognize 3 phenotypes reflecting the severity of symptoms, viz., the Hurler, Scheie and Hurler/Scheie syndromes. In this study, 10 unrelated Chinese MPS I families (nine Hurler and one Hurler/Scheie) were investigated, and 16 mutant alleles were identified. Three novel mutations in IDUA genes, one missense p.R363H (c.1088G > A) and two splice-site mutations (c.1190-1G > A and c.792+1G > T), were found. Notably, 45% (nine out of 20) and 30% (six out of 20) of the mutant alleles in the 10 families studied were c.1190-1G > A and c.792+1G > T, respectively. The novel missense mutation p.R363H was transiently expressed in CHO cells, and showed retention of 2.3% IDUA activity. Neither p.W402X nor p.Q70X associated with the Hurler phenotype, or even p.R89Q associated with the Scheie phenotype, was found in this group. Finally, it was noted that the Chinese MPS I patients proved to be characterized with a unique set of IDUA gene mutations, not only entirely different from those encountered among Europeans and Americans, but also apparently not even the same as those found in other Asian countries.